Transcriptional Regulations on the Low-Temperature-Induced Floral Transition in anOrchidaceaeSpecies,Dendrobium nobile: An Expressed Sequence Tags Analysis

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Transcriptional Regulations on the Low-Temperature-Induced Floral Transition in an Orchidaceae Species, Dendrobium nobile: An Expressed Sequence Tags Analysis

Vernalization-induced flowering is a cold-relevant adaptation in many species, but little is known about the genetic basis behind in Orchidaceae species. Here, we reported a collection of 15017 expressed sequence tags (ESTs) from the vernalized axillary buds of an Orchidaceae species, Dendrobium nobile, which were assembled for 9616 unique gene clusters. Functional enrichment analysis showed th...

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Comparative Transcriptomic Analysis of Vernalization- and Cytokinin-Induced Floral Transition in Dendrobium nobile

Vernalization is required for floral initiation in Dendrobium. Interestingly, those beneficial effects can also be achieved by exogenous cytokinin application in greenhouses. Thus, an as yet unknown crosstalk/interaction may exist between vernalization and cytokinin signaling pathways. In this study, we showed, by de novo transcriptome assembly using RNA-seq data from both vegetative and reprod...

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Analysis of gene expression associated with cold acclimation in blueberry floral buds using expressed sequence tags

To gain a better understanding of changes in gene expression associated with cold acclimation in the woody perennial blueberry (Vaccinium spp.), a genomics approach based on the analysis of expressed sequence tags (ESTs) was undertaken. Two cDNA libraries were constructed using RNA from cold acclimated and non-acclimated floral buds of the blueberry cultivar Bluecrop and about 600 5′-end ESTs w...

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Expressed sequence tags: analysis and annotation.

Expressed sequence tags (ESTs) present a special set of problems for bioinformatic analysis. They are partial and error-prone, and large datasets can have significant internal redundancy. To facilitate analysis of small EST datasets from in-house projects, we present an integrated "pipeline" of tools that take EST data from sequence trace to database submission. These tools also can be used to ...

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Expressed Sequence Tags

Now a days, genome analysis has employed a rapid analysis tool known as expressed sequence tags. In 1983, SD Putney for the first time demonstrated the use of cDNA in identification of genome. The term expressed sequence tags (ESTs) was coined by Anthony Kerlavage at the Institute for Genomic Research. In 1991, Mark Adams used EST in relation to gene discovery and Human Genome project. cDNA or ...

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ژورنال

عنوان ژورنال: Comparative and Functional Genomics

سال: 2012

ISSN: 1531-6912,1532-6268

DOI: 10.1155/2012/757801